fig. 1 —
Analytics provides the information for the quality of the product itself, as well as the levels of impurities. By providing sensitive and accurate analytical data, analytics can help upstream development by performing spent media analysis, or downstream purification by analyzing protein products.
|Subject||Characteristics||JOINN’s Analytical Methods|
|Spent Media||Expressions (titer)|
|HPLC, biosensor (Octet)|
CE-SDS, SDS-PAGE, MassSpec
MassSpec (intact, subunit, peptide mapping)
SEC-MALS, 96-well DLS, Non-reducing SDS Page
Capillary isoelectric focusing (cIEF), HPLC-IEX
Released glycan by HPLC, MassSpec for attached glycan
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